Product Info Summary
SKU: | A00021-3 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IHC |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-IL10 Antibody Picoband™
SKU/Catalog Number
A00021-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL10 Antibody Picoband™ catalog # A00021-3. Tested in ELISA, Flow Cytometry, IHC applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL10 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00021-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.005mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived rat IL10 recombinant protein (Position: K52-A170).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00021-3 is reactive to IL10 in Mouse, Rat
Applications
A00021-3 is guaranteed for ELISA, Flow Cytometry, IHC Boster Guarantee
Observed Molecular Weight
19 kDa
Calculated molecular weight
20.517kDa
Background of IL-10
Interleukin-10 (IL-10 or IL10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans IL-10 is encoded by the IL10 gene. It is capable of inhibiting synthesis of pro-inflammatory cytokines like IFN-gamma, IL-2, IL-3, TNFalpha and GM-CSF made by cells such as macrophages and regulatory T-cells.IL-10 also displays potent abilities to suppress the antigen presentation capacity of antigen presenting cells. Kim et al. (1992) showed that the mouse IL 10 gene contains 5 exons and spans about 5.2 kb of genomic DNA. Eskdale et al. (1997) mapped the IL10 gene to the junction between 1q31 and 1q32.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat
Flow Cytometry, 1-3μg/1x106 cells, Rat
Direct ELISA, 0.1-0.5μg/ml, Rat
Validation Images & Assay Conditions
Click image to see more details
Figure 1. IHC analysis of IL10 using anti-IL10 antibody (A00021-3).
IL10 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-IL10 Antibody (A00021-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 2. IHC analysis of IL10 using anti-IL10 antibody (A00021-3).
IL10 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-IL10 Antibody (A00021-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. Flow Cytometry analysis of rat spleen tissue using anti-IL10 antibody (A00021-3).
Overlay histogram showing rat spleen tissue stained with A00021-3 (Blue line).The tissue section was blocked with 10% normal goat serum. And then incubated with rabbit anti-IL10 Antibody (A00021-3, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For IL10 (Source: Uniprot.org, NCBI)
Gene Name
IL10
Full Name
Interleukin-10
Weight
20.517kDa
Superfamily
IL-10 family
Alternative Names
CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF IL10 CSIF, GVHDS, IL-10A, TGIF, IL10 interleukin 10 interleukin-10|T-cell growth inhibitory factor|cytokine synthesis inhibitory factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL10, check out the IL10 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL10 Antibody Picoband™ (A00021-3)
Hello CJ!
A00021-3 has been cited in 7 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Regional tissue immune responses after sciatic nerve injury in rats
Human interleukin-10 gene inhibits acute rejection by triggering apoptosis in allograft vascular transplantation
Myocardial ischemic post-conditioning protects the lung against myocardial ischemia/reperfusion-induced damage by activating GSK-3β
Putative role of ischemic postconditioning in a rat model of limb ischemia and reperfusion: involvement of hypoxia-inducible factor-1α expression
Inhibition of lipopolysaccharide‐mediated rat alveolar macrophage activation in vitro by antiflammin‐1
Targeted depletion of tumour-associated macrophages by an alendronate–glucomannan conjugate for cancer immunotherapy
Species: Mouse
Niu Z, Ma J, Chu H, Zhao Y, Feng W, Cheng Y. Neurosci Lett. 2012 Nov 7;529(2):112-7. Doi: 10.1016/J.Neulet.2012.09.034. Epub 2012 Sep 27. Melanocortin 4 Receptor Antagonists Attenuates Morphine Antinociceptive Tolerance, Astroglial Activation And ...
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-IL10 Antibody Picoband™?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-IL10 Antibody Picoband™
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question